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Registro Completo |
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Biblioteca(s): |
Embrapa Clima Temperado. |
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Data corrente: |
17/07/2012 |
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Data da última atualização: |
07/10/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MONTE, L. G.; CONCEIÇÃO, F. R.; COUTINHO, M. L.; SEIXAS, F. K.; SILVA, E. V. da; VASCONCELLOS, F. A.; CASTRO, L. A. S. de; HARTLEBEN, C. P.; DELLAGOSTIN, O. A.; ALEIXO, J. A. G. |
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Afiliação: |
LEONARDO G. MONTE, UFPel; FABRICIO, R. CONCEIÇÃO, UFPel; MARIANA L. COUTINHO, UFPel; FABIANA K. SEIXAS, UFPel; EVERTON F. da SILVA, UFPel; FLÁVIA A. VASCONCELLOS, UFPel; LUIS ANTONIO SUITA DE CASTRO, CPACT; CLÁUDIA P. HARTLEBEN, UFPel; ODIR A. DELLAGOSTIN, UFPel; JOSÉ A. G. ALEIXO, UFPel. |
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Título: |
Monoclonal antibodies against the leptospiral immunoglobulin-like proteins A and B conserved regions. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Comparative Immunology, Microbiology and Infectious Diseases, v. 34, n. 5, p. 441-446, set. 2011. |
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ISSN: |
0147-9571 |
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DOI: |
https://doi.org/10.1016/j.cimid.2011.08.003 |
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Idioma: |
Inglês |
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Conteúdo: |
Leptospirosis is an infectious disease caused by pathogenic spirochetes of the genus Leptospira that affects humans and a wide variety of animals. Recently the genomes of Leptospira interrogans, Leptospira borgpetersenii and Leptospira biflexa species were sequenced allowing the identification of new virulence factors involved in survival and pathogenesis of bacteria. LigA and LigB are surface-exposed bacterial adhesins whose expression is correlated with the virulence of Leptospira strains. In this study, we produced and characterized five monoclonal antibodies (MAbs) against a recombinant fragment of LigB (rLigBrep) with approximately 54 kDa that comprise the portions of LigA and LigB (domains 2?7). The 5 MAbs obtained were of the IgG1 (2) and IgG2b (3) isotypes and their affinity constants for rLigBrep ranged from 7 × 107M−1 to 4 × 108M−1. The MAbs were able to react with the native antigen on the L. interrogans, L. borgpetersenii and Leptospira noguchii surfaces by indirect immunofluorescence, immunoblotting and immunoelectron microscopy. These results demonstrate that the MAbs anti-rLigBrep can be useful to complement genetic studies and to aid studies aiming understanding the role of Lig proteins in Leptospira pathogenesis and the development of Lig-based vaccines and improved diagnostic tests for leptospirosis. |
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Palavras-Chave: |
Monoclonal. |
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Thesagro: |
Anticorpo Monoclonal; Leptospirose. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/212910/1/12-Artigo-LeonardoMonte-Medicina-Veterinaria-A1.pdf
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Marc: |
LEADER 02283naa a2200289 a 4500
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008 2011 bl uuuu u00u1 u #d
022 $a0147-9571
024 7 $ahttps://doi.org/10.1016/j.cimid.2011.08.003$2DOI
100 1 $aMONTE, L. G.
245 $aMonoclonal antibodies against the leptospiral immunoglobulin-like proteins A and B conserved regions.$h[electronic resource]
260 $c2011
520 $aLeptospirosis is an infectious disease caused by pathogenic spirochetes of the genus Leptospira that affects humans and a wide variety of animals. Recently the genomes of Leptospira interrogans, Leptospira borgpetersenii and Leptospira biflexa species were sequenced allowing the identification of new virulence factors involved in survival and pathogenesis of bacteria. LigA and LigB are surface-exposed bacterial adhesins whose expression is correlated with the virulence of Leptospira strains. In this study, we produced and characterized five monoclonal antibodies (MAbs) against a recombinant fragment of LigB (rLigBrep) with approximately 54 kDa that comprise the portions of LigA and LigB (domains 2?7). The 5 MAbs obtained were of the IgG1 (2) and IgG2b (3) isotypes and their affinity constants for rLigBrep ranged from 7 × 107M−1 to 4 × 108M−1. The MAbs were able to react with the native antigen on the L. interrogans, L. borgpetersenii and Leptospira noguchii surfaces by indirect immunofluorescence, immunoblotting and immunoelectron microscopy. These results demonstrate that the MAbs anti-rLigBrep can be useful to complement genetic studies and to aid studies aiming understanding the role of Lig proteins in Leptospira pathogenesis and the development of Lig-based vaccines and improved diagnostic tests for leptospirosis.
650 $aAnticorpo Monoclonal
650 $aLeptospirose
653 $aMonoclonal
700 1 $aCONCEIÇÃO, F. R.
700 1 $aCOUTINHO, M. L.
700 1 $aSEIXAS, F. K.
700 1 $aSILVA, E. V. da
700 1 $aVASCONCELLOS, F. A.
700 1 $aCASTRO, L. A. S. de
700 1 $aHARTLEBEN, C. P.
700 1 $aDELLAGOSTIN, O. A.
700 1 $aALEIXO, J. A. G.
773 $tComparative Immunology, Microbiology and Infectious Diseases$gv. 34, n. 5, p. 441-446, set. 2011.
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Registro original: |
Embrapa Clima Temperado (CPACT) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
21/11/2011 |
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Data da última atualização: |
28/08/2012 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
SILVA, K. da; ZILLI, J. E.; PERIN, L.; GOMES, M. de L.; BARAÚNA, A. C.; PEREIRA, G. M. D. |
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Afiliação: |
KRISLE DA SILVA, CPAF-RR; JERRI EDSON ZILLI, CPAF-RR. |
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Título: |
Caracterização genotípica de bactérias diazotróficas endofíticas isoladas de milho cultivado em Roraima. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 33., 2011, Uberlândia. Solos nos biomas brasileiros: sustentabilidade e mudanças climáticas: anais. [Uberlândia]: SBCS: UFU, ICIAG, 2011. |
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Idioma: |
Português |
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Thesagro: |
Zea Mays. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/37845/1/Krisle-Silva-resumo-1-CBCS-2011.pdf
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Marc: |
LEADER 00682nam a2200169 a 4500
001 1906551
005 2012-08-28
008 2011 bl uuuu u01u1 u #d
100 1 $aSILVA, K. da
245 $aCaracterização genotípica de bactérias diazotróficas endofíticas isoladas de milho cultivado em Roraima.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 33., 2011, Uberlândia. Solos nos biomas brasileiros: sustentabilidade e mudanças climáticas: anais. [Uberlândia]: SBCS: UFU, ICIAG$c2011
650 $aZea Mays
700 1 $aZILLI, J. E.
700 1 $aPERIN, L.
700 1 $aGOMES, M. de L.
700 1 $aBARAÚNA, A. C.
700 1 $aPEREIRA, G. M. D.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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